Figure 4

Supplemental Fig. 4. Colocalization of muNS(T1L) and beta-tubulin in cotransfected cells treated with nocodazole for one hour. CV-1 cells were cotransfected with 1.75 micrograms of pCI-M3(T1L) and 0.25 micrograms of pCI-M1(T1L) per well. Cells were treated with 10 micromolar nocodazole added at 17 h p.t., fixed at 18 h p.t., and immunostained with Oregon green-conjugated anti-muNS rabbit IgG (red) (left column) and Cy3-conjugated mouse monoclonal antibody to beta-tubulin (green) (center column). Nuclei were counterstained with DAPI (blue). Scale bar, 10 microns.